Viral Transport Medium (VTM) yog ib qho tshwj xeeb sterile kua lossis gel siv rau kev sau, khaws cia thiab kev thauj mus los ntawm cov kab mob kis.
Basic Composition
Buffered ntsev system (xws li PBS lossis Hank's tov) kom muaj isotonicity thiab ruaj khov pH, tiv thaiv kab mob los ntawm kev puas tsuaj osmotic.
Protein stabilizers (piv txwv li, BSA, gelatin, sucrose) los tiv thaiv cov kab mob thiab txo qis degradation.
Cov tshuaj tua kab mob thiab tshuaj tua kab mob (xws li gentamicin, amphotericin B) txhawm rau txo cov kab mob thiab cov kab mob fungal.
Inactivated VTM muaj lysis buffer thiab RNase inhibitors kom sai inactivate cov kab mob thaum khaws cia nucleic acids.
Tsis yog -VTM inactivated siv cell culture-raws li formulations los tswj tus kab mob kis rau kev cais thiab kab lis kev cai.
Yam khoom
Inactivated VTM: Inactivates viruses, khaws cov nucleic acids, tsis tshua muaj biosafety, ruaj khov nyob rau hauv chav tsev kub, haum rau PCR thiab molecular kuaj.
Tsis yog -VTM inactivated: tswj cov kab mob kis tsis zoo, yuav tsum tau cov saw hlau txias cia ntawm 2–8 degree, txhawb kev cais tus kab mob, tshuaj tiv thaiv kab mob thiab cov tshuaj molecular.
Muaj nyob rau hauv cov kua, gel thiab qhuav hom rau txawv tsheb thauj mus los thiab cia tej yam kev mob.
Kev ua haujlwm nta
nruj me ntsis sterile, tsis muaj exogenous microbial paug.
Zoo stability: hom inactivated tseem ruaj khov rau ob peb hnub ntawm chav tsev kub; non- hom inactivated ruaj khov rau 48–72 teev ntawm 2–8℃.
Tau tshaj PCR, kuaj antigen, gene sequencing thiab viral cais.
Leak- qauv tsim kom muaj kev nyab xeeb kev thauj mus los.
Daim ntawv thov
Clinical Viral Detection
Cov kab mob ua pa xws li mob npaws, RSV, adenovirus thiab lwm yam kab mob.
Lwm yam kab mob xws li kab mob tes, ko taw thiab qhov ncauj, herpes, kab mob siab thiab lwm yam kab mob.
Tau tshaj oropharyngeal swabs, nasopharyngeal swabs, hnoos qeev, lavage kua thiab lwm yam qauv.
Public Health thiab Tswj Kab Mob
Loj - kev tshuaj ntsuam xyuas, kev tshawb nrhiav kab mob sib kis, chaw nres nkoj cais tawm thiab cov lus teb tshwm sim.
Kev saib xyuas tus kab mob ib puag ncig rau cov khaub thuas- cov khoom lag luam thiab cov qauv saum npoo.
Kev tshawb fawb thiab kev soj ntsuam
Kev cais tus kab mob, kev txheeb xyuas tus kab mob, kev tshawb fawb tshuaj tiv thaiv thiab kev tshawb fawb serological.
Kev soj ntsuam tus kab mob tsiaj hauv tsiaj txhu, nqaij qaib thiab tsiaj qus.
Kev xeem teb thiab zej zog
Tsim nyog rau cov hauv paus hauv paus thiab kev ua piv txwv hauv qhov chaw txias txias yog txwv.
Siv nyob rau hauv cov khoom siv kuaj hauv tsev thiab taw tes- ntawm- cov tshuab tshuaj ntsuam xyuas.
Cov txheej txheem xaiv
- Rau PCR thiab kev ntsuas molecular: xaiv qhov tsis siv neeg VTM rau kev nyab xeeb thiab chav - qhov kub thiab txias.
- Rau kev cais tus kab mob, kab lis kev cai lossis tshuaj tiv thaiv kab mob: siv tsis yog -VTM inactivated nrog 2-8℃txias saw.
- Rau kev thauj mus los ntev -nyob deb: xaiv gel- hom lossis VTM kaw ntom nti kom tiv thaiv kom tsis txhob yaig thiab volatilization.
Cim npe nrov: Tuam Tshoj virus thauj nruab nrab manufacturers, hoobkas
